Mouse anti-Human IgG (Fc Region) Antibody

Catalog No. ABIN302085

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (ABIN302085)

Target: IgG

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: EM-07

Isotype: IgG1

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Purpose: Anti-Hu IgG (Fc) Purified

Specificity: The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

Cross-Reactivity (Details): Human, Other not determined. The antibody EM-07 does not crossreact with IgM.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Fusion protein of human IgG Fc fragment.

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details

Target: IgG

Target Type: Antibody

Background: Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment

Molecular Weight: 150 kDa